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The ultrafiltration-based affinity MS technique has been established to search for ligands, verify binding and estimate affinity of specific ligands for given soluble protein targets (Chen et al. 2015; Qin et al. 2015).
To deplete BLyS in vivo, groups of 3 mice that had previously received huBLyS for 7 days were given soluble TACI-Ig (10 ug i.p./mouse/day, Human Genome Sciences) for 7 subsequent days.
To investigate the first step of the proposed mechanism, we examine the degree to which changing the number of charges in a given soluble loop shifts the position of that loop in the EOT ensemble.
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12F-FBE reacts with diphenols to give soluble, hydrophobic, low dielectric (2.30 2.43 at 10 GHz) polyethers.
BCDA gives soluble polyimides with diamines containing at least one flexible linkage such as ether, methylene and sulfide.
Bis-nadimides gave crosslinked insoluble materials and bis-tetrahydrophthalimides gave soluble materials in spite of the disappearance of their olefinic protons.
1-Ethynyl-2-phenylethynylbenzene (2) was polymerized by W and Mo catalysts to give soluble polymers with Mn of 6300 71,900 in good yields.
In particular, the methods to obtain so-called proteinlike copolymers (which give soluble globules with core-shell structure) and molecular dispensers (which are able to absorb selectively nanoparticles of a given size) are discussed.
As a result, OTSH, having the longest alkyl group among examined, could give soluble polymers, whereas other TSHs could not due to the shorter alkyl chains insufficient to overcome these factors.
Although several different forms of TβRII were expressed, only a fusion with glutathione S-transferase gave soluble TβRII, which was purified at a yield of 0.1 mg/10 L of bacterial growth.
When the concentrations of EGDMA, NMMAm and MAIB were 0.15, 0.50 and 0.35 mol/l, the copolymerization proceeded homogeneously with no gelation at 80 °C to give soluble copolymer in a yield of 50%.
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