Sentence examples for given pcr from inspiring English sources
This required that the results from at least two separate PCR experiments agree with each other for a given PCR reaction, and the results from at least two junctions (out of two insertion junctions and sometimes one pre-insertion junction) agree with each other for a given locus (except 3 loci where we were only able to design primers for one junction, see Table s1 in Additional file 1).
Expert staff from NIHRD then trained staff in regional and subregional laboratories in PCR techniques based on the protocol developed by WHO. 8 Since 2008, NIHRD has routinely given PCR training to all laboratories.
Typically, the theoretical analytical sensitivity of a given PCR with certainty is approximately 3 copies [39], assuming a Poisson distribution, and a 95% chance that at least 1 copy is detected in the PCR.
PCRs were performed in triplicate to minimize the inherent amplification bias of any given PCR of multicopy loci.
If num_restriction_enzymes = 1, then a given PCR product may be digested by only one enzyme at a time before electrophoresis or other empirical measurement of fragment lengths.
The amount of the target DNA in a given PCR tube was inferred from the number of cycles required to reach a threshold level of SYBR Green fluorescence.
The rise in P. knowlesi notifications in Sabah may also be underestimated given PCR-confirmed P. knowlesi is misdiagnosed as P. falciparum or P. vivax in up to 13%and10%0% of cases, respectively, by routine microscopic examination in this area.
The primers, matK2.1a and matK3.2r reported by Plant Working Group failed to give PCR amplification in Berberis even after changing some PCR conditions including addition of DMSO.
Indel loci with minimum putative lengths of 250 - 2500 bp were chosen for validation to give PCR amplicons with convenient product sizes.
PCR experiments with primers for the blaIMI-1 gene were positive with plasmid DNA of each E. asburiae isolate and transconjugants as templates, whereas primers designed to amplify blaNmcA and blaSme-1/Sme-2 failed to give PCR product.
Only BAC clones from one of the two contigs gave PCR-products, thus this contig was defined as the IA region, and the other contig remained a candidate for the duplicated IB region.
