As such, the only real marker of how many people are partaking in any given modification is how many people write in to thank the mod's developer or otherwise comment on their results — which is usually, at most, a few hundred people.
The aim is to change (increase or reduce) the edge lengths at minimum total cost with respect to given modification bounds such that a prespecified vertex s becomes an absolute (or a vertex) 1-center under the new edge lengths.
A further complication is the propensity of ubiquitination complexes to generate chains of varying lengths at a given modification site in vivo, making detection of ubiquitination by traditional size separation techniques challenging.
As previously observed [ 16, 17], the kinetic signatures for a given modification varied slightly depending on the surrounding sequence context.
In these cases, the relative abundances of the MS/MS fragment ions characteristic for a given modification site were used to calculate the DMBNHS modification rate constant.
By using affinity-purified autoantibodies against post-translationally modified proteins, it should be feasible to capture a spectrum of antigens expressing a given modification.
InsPecT describes an unrestrictive PTM search algorithm that searches for any possible type of modification at once in a "blind search" mode, which does not depend on any given modification list.
For our study, we looked for regions that overlap ≥ 1 nucleotide since SYT-SSX2 interacts with large protein complexes, and a 1-base overlap suggests close proximity to a given modification.
