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He was committed to a mental institution and given EST - something he believed his mother could have saved him from.
It is therefore relatively facile to determine if a given EST dataset (particularly an insect gut dataset) is indeed contaminated by transcripts from another eukaryotic organism.
* Percentage of a given EST in all ESTs obtained from that of cDNA libraries.
Only the best hits were recorded for any given EST unigene.
The cluster size varied from one to 105 copies of any given EST.
When there were multiple hits for a given EST, the hit with greatest identity and coverage was retained.
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The value of PIC was calculated using the formula where P i is the frequency of an individual genotype generated by a given EST-SSR primer pair and summation extends over n alleles.
The value of PIC was calculated using the formula PIC = 1 + ∑ i = 1 n P i 2 where Pi is the frequency of an individual genotype generated by a given EST-SSR primer pair and summation extends over n alleles.
In contrast, summation of EST counts from all different isoforms of these proteins gives EST ratios of: ELC: RLC (26:124), ACT: TPM: Tn (345:249:98), TnC I T (18 160 71) and PM MHC (18 8), which differ substantially from the protein stoichiometries.
These animals were of different ages and stages of development and the tissues that were collected are listed in Additional file 6. Tissue was sent to Genesis Biotechnology (Auckland, New Zealand) or Life Technologies Carlsbadd, California) where cDNA clones were generated and single pass sequenced giving ESTs.
If available for a given species, EST data from the NCBI EST database has been analysed to help in the annotation process.
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