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Given a mass of 206 Da = 34 pN⋅ps2/Å for the anchor residues, this gives an unfolding force of Funfold = 2 M Vcritical = 2×34×2.2 = 177 pN, which is close to the measured unfolding force of 203±35 pN [21].
Given a mass of 262 Da = 44 pN⋅ps2/Å for the anchor residues, this gives an unfolding force of Funfold = 2 M Vcritical = 2×44×1.8 = 158 pN, which is close to the measured value of 182±5 pN [21].
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The (metric) unit of pressure in the scientific international system of units (known as the SI system) is newton per square metre (N/m2), where one newton (N) is the force that gives a mass of one kilogram an acceleration of 1 m/s2.
Gravity measurements give a mass of 10 billion tonnes.
MALDI-TOF mass spectrometry analysis gave a mass of 6160.03 (Fig. 2f) that is accord with the predicted molecular weight.
This method gave a mass of precipitate of 30.2 mg, a value higher than the estimate determined by dissolving the precipitate during post-mortem analysis of the same diffusion cell (21.7 ± 0.4 mg).
Human proinsulin containing an N-terminal methionine, M-proinsulin, was engineered and converted into a perdeuterio derivative, which using an optimized mass spectrometric protocol and manual calculations gave a mass of 9669.6 (± 1) Da showing the replacement, with deuterium of 146.4 from a total of 149 exchangeable hydrogen atoms (83 from amides and 66 from side-chains).
The knob is varying densities of cancellous bone with a relatively thin layer of compact bone, and is here modeled as cancellous bone (1 giving, giving a mass of 2010 g.
The major peak gave a mass of 47007.4 Da and likely encompasses the 50 kDa band.
Electrospray ionization (ESI) mass spectrometry analysis gave a mass of 20,758 Da that matched the theoretical mass (20,757 Da) of SUMO-Flag-TibA305-350-Flag.
Combining the Rs = 7.6 nm (from 1/ D = 0.35 × 10-7, and S = 7.4, Eq. 7.1b gives a mass of M = 236 kDa, close to the 204 kDa obtained from adding the mass of the ten subunits. Smax is 12.6 giving Smax /S = 1.7, suggesting a moderately elongated protein. Figure 6 shows EM images of DASH/DAM1 by rotary shadowing (a) and negative stain (b).
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Justyna Jupowicz-Kozak
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