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To enhance the relative production of fully elaborated rapalogs, genes encoding late-acting biosynthetic pathway enzymes which failed to act efficiently on the novel compounds were expressed ectopically to give strain BIOT-4110.
The resulting plasmid, pJG32, was transformed into E. coli strain BL21 to give strain JG73.
The torAhis allele was then moved on to the chromosome of strain DSS401 (as MC4100, Δ dmsABC::KanR) [ 25] to give strain FTH007.
The chromosomally integrated transgenic array leIs2703[ceh-19b prom:: gfp, unc-119] was crossed from strain UL2703 into the ceh-19 tm452) ceh-19 tm452round of UL3128 to give strain UL3413.
To avoid the polar effect on the cdsA expression, a xylose-inducible promoter (P xyl ) was inserted in front of the cdsA gene to give strain YK1889 (Δ uppS:: kan P xyl - cdsA pLOSS- P spac - uppS).
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Studying plants of a given strain under multiple environments can greatly help to reveal their interactions.
For a given strain magnitude, the dissipation will be enhanced as the loading rate increases.
Large differences were found in the mechanical responses during first loading to a given strain.
Use of the equivalent stress intensity factor allowed prediction of the crack growth for a given strain range.
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