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This allowed for the consistent use of standardized thermal cycling conditions performed using a Mx3000P Real-Time PCR System (Stratagene): 95 °C for 10 min, followed by 40 cycles of 95 °C for 30 s, 60 °C for 1 min and 72 °C for 1 min which were found to give efficiencies > 90%% (see below).
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We also give efficiency factors of the usual binary block neighbour balanced designs which can be used when the number of blocks is small.
Calculations done will be made based on the given efficiencies assuming that the whole process is described including the chemical treatment, necessary pumps and further equipment.
We used the primers that gave efficiency close to 100%.
For example, one division may be less efficient on average, but the other may be more costly so that a given efficiency improvement is more beneficial.
The optimal ingesta retention time for a herbivore of a given efficiency in particle size reduction will always be limited by the maximum digestibility of the forage it feeds on.
Assays with the observed consistent amplification of duplicates on a standard curve (R2 > 0.95) giving efficiency values of 2.0 (within 15%) were considered acceptable for normalization and expression analysis.
The pan was found to give higher efficiencies in all cookstoves.
- Reach efficiency gives the percentage of all secondary contacts divided by size.
The range of speed for which hydraulic turbines give acceptable efficiency is much lower than for steam turbines.
"I will give this efficiency review my very best effort, knowing how hugely important it is to the recovery of the country.
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