Too many yeast cells in a tranformation mix will significantly drop the efficiency and will give difficulties to spread on the plates.
Therefore using measures gained solely by clinical professionals could be seen as unreliable given the difficulty to train and calibrate on a given index.
Although, in the finale of the Ninth Symphony and the Missa Solemnis, Beethoven shows himself a master of choral effects, the solo human voice gave him difficulty to the end.
Given the difficulty to maintain sensors in deep locations, a particular emphasis is put on observer-based control design.
Given the difficulty to obtain phosphorylated or acetylated PKM2 proteins, we purified PKM2Y105E (a phosphorylation mimic of Y105) and PKM2K305Q (an acetylation mimic of K305) to mimic PKM2 containing the two modifications.
This cascaded CNN architecture tends to learn well from a small (n≤35) set of labeled data of the same MRI contrast, which can be very interesting in practice, given the difficulty to obtain manual label annotations and the large amount of available unlabeled Magnetic Resonance Imaging (MRI) data.
Given the difficulty to therapeutically decrease HIF-1α protein stability in tumors, most drug discovery efforts focus on inhibiting HIF transcriptional activity [e.g. ref.19].
Given the difficulty to identify emotions in alexithymia, we further hypothesized a reduced sensitivity to affective mismatches in alexithymia, reflected in diminished N400 amplitudes in response to affectively incongruent compared to congruent conditions as a function of alexithymia.
The spontaneous component of neuropathic pain (NP) has not been explored sufficiently with neuroimaging techniques, given the difficulty to coax out the brain components that sustain background ongoing pain.
29 Therefore, there is a compelling need to identify biomarkers for the diagnosis and the prediction of surgical outcome, given the difficulty to detect heart failure in this complex group of patients.
