Sentence examples for gg were used from inspiring English sources

For the detection of the wild type allele the oligonucleotides AE3wt.for 5'-GAT GAA GAT GAC AGC CCA GGC CTT CC, and AE3wt.rev 5'-CCG GCT CTT CTG TGT GGA GAT TCG GG, were used as forward and reverse primers, and amplified a 593 bp fragment.

Genomic DNAs from HapMap cell lines with known genotype (AA, AG and GG) were used as controls.

In this study, the groundnut husk modified with Guar Gum (GG) is used for the removal of Pb2+, Cu2+ and Ni2+ from the aqueous solution.

The T7 primer (5' TAA TAC GAC TCA CTA TAG GG 3') was used as the "forward" primer and the BES_HR primer (5' CAC TCA TTA GGC ACC CCA 3') was used as the "reverse" primer.

A 'typical' rs4072037 AG heterozygote and an rs4072037 AA and rs4072037 GG homozygote were used as controls.

Hsp 70 specific primers (forward 5′-GAT CAC CAT CAC CAA CGA C-3′ and reverse primer 5′-ACT TGT CCA GCA CCT TCT TC-3′) and Hsp 27 specific primers (forward 5′-TGC TTC ACC CGG AAA TAC AC-3′ and reverse primer 5′-CTC GAA AGT AAC CGG AAT GG -3′) were used.

CH1 for (5′-GTC CTT GAC CAG GCA GCC CAG GGC-3′) and M13 reverse (5′-AGC GGA TAA CAA TTT CAC ACA GG-3′) were used as forward and reverse primers, respectively.

Two pairs of primers, Bat_Cytb_1 (5′-TAG ATC AGC AGC TTT GGG TG-3′) (21 ) with Bat_Cytb_2 (5′-AAA TCCGCCG TTG TAC TTC AAC-3′) (21 ), and Bat_Cytb_2 with BAT15R (5′-TCA GCTTGTGGTGTTGAT GAT GGG-3′ (22 ), were used because of amplification specificity of certain primers in some species.

The powder forms of SM, GG, and WS were used in the screening experiment to choose the most suitable substrate for maximum α-Gal production.

To exclude technical problems in group (3) samples, we also used a crude telomerase extract in the TRAP assay (supplementary fig. S3 A, Supplementary Material online) and alternative substrate primers (47F, GG(21), CAMV) were used to meet possible different substrate sequence requirements for the telomerase action.

Reactions were performed according to standard protocols using the following primers and conditions: for Cox-2 (GenBank: AJ634912.1) forward 5′-AGC TGG GAA GCC TTC TCT AAC-3′ and reverse 5′-AGA TCA TCT CTG CCT GAG TAT CTT-3′ and for β-actin (as internal control; GenBank: X00351) forward 5′-GAT GAT GAT ATC GCC GCG CT-3′ and reverse 5′-CTT CTC GCG GTT GGC CTT GG-3′ primers were used.