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The results showed that when the 30Kc19 protein is fused with GFP, it was able to penetrate and deliver its cargo protein into cells (Fig. 7).
By fusing Hd3a or RFT1 with GFP, it was demonstrated that Hd3a or RFT1 protein was expressed in vascular tissue of leaves, and could be moved to SAM where they started flowering induction.
For wild-type GFP, it has been reported that the fluorescence is unaltered in the range of pH 6 - 10 but decreases at lower pH and increases at pH values > 10 [29].
Since PI emission wavelength overlaps with that of GFP, it was impossible to distinguish the proliferative/survival events within GFP+ cell population.
Thus, since the majority of the GFP- tagged-Kb tumor cells weekly expressed GFP, it is possible that after DC uptake and display, the relatively small numbers of Kb molecules/peptide complexes that were transferred were difficult to observe with fluorescence microscopy techniques.
When the lysates from individually transfected wells were subjected to SDS-PAGE and Western blotting, and the blots were probed with an antibody against GFP, it was found that the proteins were expressed at the same levels (Figure 5A) indicating no differences in the stability of the two proteins.
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Similarly, when we monitored the PI 4,5 P2 reporter PH-PLCδ-GFP, it remained apically enriched following co-expression with a Rho1 RNAi construct, indicating that Rho1 does not directly regulate PI 4,5 P2 distribution.
Although the total number of Nuf2 molecules per kinetochore remained almost constant for lower expression values of Nuf2-GFP, it increased for high expression of Nuf2-GFP (Fig. 5e, last four data points).
If the mammary gland that grew did not express GFP, then it was residual host mammary gland (GFP negative).
GFP, as it is known to its friends, gives the glow to Aequorea victoria, a jellyfish that lives in the eastern Pacific Ocean.
Its structure is similar to GFP, but it is tetrameric.
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