Exact(9)
As controls, we used two clones, GFP #1 and GFP #3, which have no inducible TAM67 expression.
Embryos were microinjected with mDUX plus GFP (1 ng+100 pg) or GFP (1 ng) mRNA alone at the four-cell or sixteen-cell stage (NF) embryos.
GFP 1 corresponds to amino-acid residues 174 238 and GFP2 to amino-acid residues 1 173 in GFPi 9/8 topology.
TAM67 #8 and GFP #1 were injected subcutaneously into nude mice.
Similarly, the tumours from GFP #1 grew fast in the absence and presence of doxycycline.
The induction of GFP did not show such effects in GFP #1.
Similar(51)
Primary antibodies used were: V5 (1∶500, Invitrogen), GFP (1∶1000, Abeam, Cambridge, MA).
Cy3NTA quenched GFP(1 DR3His to a similar extent (20.1%) compared to GFP-RyR1 -His) GFP-RyR1 -HisA quenched GFP-RyR1 -Hiss by 52.7% (Fig. 7b).
A performance gap has been noticed for natural RFPs after isolation from marine invertebrates [2] [4], in comparison to variants of the classical green fluorescent protein (GFP) [1].
Membranes were probed with antibodies against GFP 1∶5000 (Abcam; Abcam) or C. elegans γ-tubulin 1∶1000 (ab50721;Abcam) overnight in blocking solution.
Primary antibodies against ChAT (1∶200, goat polyclonal, Millipore), GFP (1∶1000, rabbit polyclonal; Molecular Probes, Eugene, OR), GFP (1∶500, goat polyclonal; Rockland Immunochemicals, Gilbertsville, PA), and CtBP2 (RIBEYE) (2 µg/ml, rabbit polyclonal; BD Biosciences, San Jose, CA) were used.
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