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This method may be subject to selection bias (i.e. in general, only important or controversial results get replication attempts in the first place).
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A few teams, including one led by Floyd Romesberg, a biological chemist at the Scripps Research Institute in San Diego, California, have even managed to get DNA replication proteins called DNA polymerases to copy DNA strands incorporating alternative letters.
"What we'll get is a replication of the Obama stimulus package in 2009".
In order to get high virus replication in pigs and generate PRRSV (Lena) quasispecies which might favorably contribute to the adaptation on MARC-145 cells, two 4-week-old piglets from a PRRSV-negative farm were inoculated oronasally with 105 TCID50/pig of macrophage-grown virus.
The ways to ensure more robust results include using family-based samples and/or getting independent replications.
Mr. Rodgers and Mr. Williams both appear on the album's first single, "Get Lucky," an uncanny replication of the sparkling disco-funk of Mr. Rodgers's band Chic.
That's exactly where you get this problem of replication of bias.
In the first GWAS presented on obesity, this gene was found to be associated, but did not get validated in the replication stage [ 53].
Single round viral replication is get by infecting RD cell at multiplicity of infection of 5, collecting at 16 h post infection, and testing culture supernatant and intracellular RNA at 16 h post infection.
It's difficult to get funding to do replications, and if you manage to replicate a finding, journal editors are likely to dismiss it ("What does this add to the literature?" they say).
Instead, we run multiple independent replications to get a uniform distribution of UEs over the simulated area.
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CEO of Professional Science Editing for Scientists @ prosciediting.com