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To get colony area and the number of cells per colony, the Morphology Explorer™ image analysis algorithm available through the ArrayscanVTI software was used.
To get Colony forming unit-fibroblast (CFU-F) single cell BMC suspensions were diluted to a concentration of 2.5×106 cells/ml in DMEM supplemented with 10% FCS and plated in 24-wells plates.
Bacteria live or heat-killed (60 °C for 1 h) were washed with phosphate-buffered saline (PBS, 0.02 M, pH 7.2) and counted by the spectrophotometric method and confirmed by pour plate colony counts to get colony forming units (CFU).
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"Fundamentally it came because imperialists invaded to get colonies.
"Mobility is extremely limited, and beekeepers can't get colonies to the citrus groves where they're needed, or to the lush areas in Kurdistan".
The bee nerds finally got "Colony Collapse Disorder".
He has issued a wake-up call: To ensure our long-term survival, we need to get a colony up and running on Mars within 46 years.
during World War II to defeat Japan, but as soon as the war ended the United States sided with France in its desire to get its colony back.
Paice doesn't speculate, but I suspect von Lettow-Vorbeck was more reactionary conservative than Nazi, though he did keep campaigning for Germany to get its colonies back.
The fraction of untransformed cells emerging within each culture was calculated by plating appropriate dilutions of this culture onto LB plates (without antibiotics) to get isolated colonies.
Cells from each genotype of interest were streaked onto YPD plates for 2 days at 30°C to get individual colonies.
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