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This leads us to believe that unless very large numbers of sequences are produced, the use of microsatellite enrichment strategies [ 17, 50, 51] will be the most productive way for cultivated germplasm marker development.
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but only the additional information about the germplasm the marker was tested on.
In the past decades, major R genes, including Xa7 and Pi2, have been frequently introgressed or pyramided into modern adapted or elite rice genetic backgrounds from exotic germplasm via marker-assist backcrossing (Huang et al. 2012; Jiang et al. 2012).
Moreover, information about which germplasm the markers have been tested on (resulting from the genotyping activity conducted within the CEREALAB project) are available in the class Marker_tested_on_Germplasm.
Functional genomics studies have identified potential candidate genes and regulatory factors for adaptation, yield and quality traits, which could be introgressed at an accelerated rate into elite germplasm by marker-assisted breeding or directly engineered into economically important crop plants.
Past efforts led to believe that low molecular variation exists in cultivated chickpea; however, this conclusion is based on limited number of germplasm and markers involved in these studies.
Owing to their codominant nature and ease of transferability between germplasm, microsatellite markers (simple sequence repeats; SSRs), have found utility for a variety of purposes including the development of transferable, saturated linkage maps in many rosaceous genera [ 17- 19], and SSRs have recently been developed for Rubus [ 20- 22].
Several studies have been conducted to understand the genetic diversity of pearl millet germplasm using RFLP markers [ 27], AFLP markers [ 14, 28], and SSR markers [ 15, 29– 329.
The data gathered here demonstrates the feasibility of genotyping extensive germplasm collections at marker loci in rice genome using panels of microsatellite markers, each of them multiplexed with a single PCR assay prior to electrophoresis.
In addition, they are broadly used in crop and livestock breeding for germplasm characterization and marker assisted selection [ 8].
In order to assess the potential of newly developed markers for germplasm analysis, screening of these markers on 32 genotypes showed reasonable level of polymorphism.
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