Additionally, the adoption of common high-density SNP genotyping assays will also greatly facilitate the comparison of QTL regions across studies, germplasm and laboratories.
The success of non-contaminant control methods based on genetic strategies depends on compatibility between natural and laboratory germplasms.
One main cause for the success of non-contaminant control programs based on genetic strategies is compatibility between natural and laboratory germplasms.
Samples were selected and purified by researchers working in the Laboratory of Germplasm Improvements and Applications of Pyropia in Jimei University, Fujian Province, China.
However, testing of the RAPD and SCAR primers in a P. vera (Siirt × Bağyolu) F1 segregating population, and in a large germplasm collection in our laboratory, revealed false negatives in some female individuals and false positives in some male individuals, including the Bağyolu male parent.
The authors thank Professor Yuepeng Han (Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences) for critical review of this manuscript.
If the laboratories obtain equivalent results for these samples, then the distribution of values in the database may represent true differences in the analyte concentrations between the germplasm sources sent to each laboratory.
In vitro storage refers to the storage of germplasm in aseptic culture under laboratory conditions, while cryopreservation refers to the storage of cells, tissues, seeds, etc. at temperatures of liquid nitrogen (-196ºC).
During the last two decades, studies by different laboratories using diverse germplasm led the development of several molecular markers and genetic linkage maps besides mapping quantitative trait loci (QTL) for erucic acid, glucosinolates, disease resistance, plant morphology, flowering time, and yield component traits.
Reference strain A. hydrophila strain ATCC 7966 (store in Key Laboratory of Freshwater Fishery Germplasm Resources, Shanghai) was used for quality control.
The cereal broad-based germplasm collections maintained by the CEREALAB laboratory comprise about 400 rice, 70 barley and 150 bread wheat accessions, containing a significant sample of genetic variation.
