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The response of F-spores to germinants was measured in terms of both fluorogenic ability and loss of optical density, a traditional parameter for quantitative measurements of germination rate based on rapid reduction of the spore's refractive index immediately after breaking dormancy [14], [15].
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Germination rates were scored based on radicle protrusion.
Germination rates were scored based on radicle protrusion after three days of stratification.
Each replication represents a pool of germinating barleys that were carefully selected from an independent germination experiment based on the relative time point and morphology defined for the given stage to reduce the heterogeneity of the pooled barley tissues potentially caused by their different germination rate.
Further, we estimated the in vitro pollen performance of each pollen donor by assessing pollen tube growth rate in Hoekstra germination medium [75], based on a previously observed correlation between in vivo and in vitro pollen tube growth in this species [76].
The rate is based on availability.
Spore germination was evaluated based on the decrease in OD580 at room temperature.
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Rates are based on availability.
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