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For all studies, seeds were germinated for 4-6 days on germination paper and then planted in hydroponic media per methods described in Grusak et al. [ 25].
Lowest % synersis value and least gelation concentrations were observed in native sorghum has, which increased during germination and were highest in sorghum germinated for 48 h at 35 °C.
Maize seeds were surface-sterilized with 0.5% (w/v) NaOCl for 5 minutes, thoroughly rinsed in deionized water and germinated for four days on moistened germination paper rolls.
A Sudanese sorghum cultivar (Fetarita) was germinated for five days and protease and amylase activities were measured every 24 h.
When these spores are germinated for use in analytical techniques, they show no loss in performance, even after long periods of storage under harsh conditions.
For phosphate treatment experiments, seeds were germinated for 5 days on vertical 1/4th MS (Murashige and Skoog) Plates (supplemented with 1% agar, at pH 5.7) in growth room (22 °C; continuous light; 100 125 µmol/m2/s).
Sorghum was germinated for different time (12, 24, 36 and 48 h) at different temperatures (25, 30 and 35 °C) and the changes in their nutritional and functional properties of germinated sorghum flour were assessed and compared with native sorghum flour.
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The seeds of each rice resistance-improved varieties and susceptible varieties were pre-germinated for the identical developmental stage.
Gas-sterilized and cold-treated seeds were pre-germinated for 36 hr on half-strength Murashige and Skoog basal salts medium (Sigma-Aldrich, USA), pH 5.8, plus 1% sucrose, 0.5 g/L MES (Merck, Germany) and 1.5% agar.
In a quest for submergence tolerance at the seedling stage, Yamauchi et al. (1993) screened 258 accessions from the International Rice Research Institute (IRRI) gene bank and 404 from the International Network for the Genetic Evaluation of Rice (INGER), using seeds pre-germinated for 2 days and then sown at 25-mm soil depth and submerged with 20 50 mm of water.
A. fumigatus conidia were germinated for 12 h in RPMI 1640 with and without caspofungin.
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