Sentence examples for germ systems from inspiring English sources

Comparing protein solubility in E. coli bacteria and the cell-free E. coli and wheat germ systems, we found that the wheat germ system produces the highest solubility rate (97%).

In vitro translation analysis was performed using a coupled transcription/translation wheat germ system (Promega).

We found no difference between C- or N-terminal tag in the wheat germ system.

We co-expressed the rAahII toxin in the wheat germ system with hQSOX1b and hPDI, respectively.

Obviously, the expression of proteins with molecular weights higher than 70 kDa are critical for the wheat germ system [ 22].

Reaction life-time and productivity of the cell-free wheat germ system was increased by the introduction of a dialysis system to facilitate removal of inhibitory by-products.

These targets were subjected to a more detailed analysis comparing a prokaryotic cell-free E. coli system with an eukaryotic wheat germ system.

However, 6 open reading frames did not express in the wheat germ system, but were previously successfully expressed in vitro in E. coli (Table 2).

20 Despite various high-throughput applications for the wheat germ system, the functionality of synthesized proteins is a key aspect for downstream analysis.

In the wheat germ system, 65 were expressed using the N-terminal wild type construct (75%) and 67 with the C-terminal tag (78%).

Thus, for in vitro protein expression, specifically for toxic proteins which can not be expressed in bacteria, the wheat germ system is the method of choice.