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Since the East Coast anthrax scare last fall, many researchers have worried that a broader use of Cipro, a drug of last resort against powerful infections, would engender resistant germ strains.
In a report scheduled for release next month, the state's Little Hoover Commission describes a 30-year erosion of a health system that has left Californians vulnerable to disease outbreaks, new germ strains, hospital-acquired infections and widespread poisoning.
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It has also bred 5011 mutant mouse strains that have germ line transmission of the knockout.
After injection of recombinant ES cells into B6 blastocysts, chimeras were crossed to the B6 strain to identify germ line transmission.
DNA damage in the C. elegans germ line was assessed with the strain hus-1 gfp hus-1 gfped previously.
Germ line retroviruses in certain inbred mouse strains replicate and infect other cells within the hematopoietic system.
Similar results were observed using animals carrying a mutation in the maternal effect gene pgl-1, which is required for postembryonic germ line development, and the germ line-deficient strains glp-1 and glp-4.
Transgenic strains expressing kinetochore proteins fused to fluorescent proteins were generated by standard P-element-mediated germ line transformation.
Obtaining a mouse strain that carries the gene-targeted mutation requires breeding the chimeras to transmit the ES cell genetic component through the germ line to the next (F1) generation (germ line transmission, GLT).
Additional studies indicated that the FOXO transcription factor DAF-16 is required for resistance to P. aeruginosa-induced neurodegeneration in germ line-deficient strains.
Furthermore, the most lethal form of the germ is the A strain, while the form found by the ISG was the B strain.
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