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Because the task force has two expensive, highly sophisticated, transportable labs in which chemical and germ samples can be analyzed quickly, it was kept at a safe distance from fighting at a desert camp in Kuwait, just across the Iraqi border.
She pointed out an experiment that involved feeding a form of Vitamin E to fruit flies to see if it made them live longer, and one that involved collecting germ samples from the hands of the school's nurses and custodians to determine whether various cleaning agents killed the bacteria.
Flaking grits and germ samples were obtained from a commercial corn dry-milling plant (Bunge, Danville, IL, USA).
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To generate the corresponding mRNA transcriptome we have sequenced Illumina paired end TruSeq libraries from the same N. benthamiana leaf, stem, germ, root, flower and seed samples as at the small RNAs.
Concerning the mouse, this dataset includes one somatic testicular sample (Sertoli cells), three purified germ cell samples (spermatogonia, pachytene spermatocytes and round spermatids) as well as seminiferous tubules and whole testis whose RNAs were hybridized to Mouse430_2 Affymetrix GeneChips (Figure 1 and Tables S1 and S2).
With respect to the human dataset, two purified germ cell samples (pachytene spermatocytes and round spermatids), seminiferous tubules and whole testis were hybridized to HG-U133_Plus_2 GeneChips (Tables S1 and S2).
Although sorted germ cell samples removed most somatic cells, their expression patterns may have changed dramatically from in vivo status.
These analyses highlighted several network hubs, including miRNA clusters and stem cell genes that distinguish YST from normal germ cell samples and other GCTs.
This may be because previous studies have focused only on methylation of gene promoters and not broader genomic contexts in germ cell samples.
The correlation observed between isolated germ cell samples and total testes harbouring active spermatogenesis is not surprising given the large number of differentiating germ cells that accumulate during testis maturation.
At least 3 clusters corresponding to genes preferentially-expressed in somatic cells, as evidenced by their low or null expression in isolated germ cell samples, could thus be distinguished.
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