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This is consistent with previous TRALI models, in which both a clinical first event, either LPS-infusion or, in the case of mice, their exposure to a germ environment, and an appropriate second event (that is, stored blood or leucocyte antibody) were required for TRALI to develop [ 9, 10, 12, 38, 39].
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All mice were maintained in a germ free environment and housed in accordance with the Animal Welfare Act in facilities accredited by the AAALAC (at UMDNJ-New Jersey Medical School). UMDNJ-New Jersey Medical School
They placed some diabetes-susceptible mice in a germ-free environment and exposed others to only those bacteria.
Sonnenburg and her colleagues raised mice in a germ-free environment and then fed them human feces, giving them human gut bacteria.
Bäckhed and colleagues started with mice raised in a germ-free environment and exposed some of them to a variety of microbes from the gut of another mouse.
Those were infused into the stomachs of mice raised in a germ-free environment and, therefore, without a gut microbiome of their own.
Nude (nu/nu) mice and Sprague-Dawley rats (both from Charles River, Wilmington, MA, USA) were maintained in a germ-free environment and had access to food and water ad libitum.
Man had not put the germs into the environment, and his role in spreading them was involuntary.
In the Salon d'Automne of 1922, Le Corbusier exhibited two projects that expressed his idea of social environment and contained the germ of all the works of this period.
Some of the rodents were brought up in a sterile environment and thus were germ-free, with no detectable microbes in their bodies.
Athymic nude mice (nu/nu; 48 female, 5 7 weeks old) were divided into 8 treatment groups with 6 animals in each group and maintained within a specific pathogen and germ-free environment.
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