Exact(23)
Many insights into mammalian germ cell development have been gained through genetic engineering and in vivo studies, but the lack of an in vitro system for deriving germ cells has hindered potential advances in germ cell biology.
Indeed, analysis of sperm at 28 + 3d has generated negative results with established germ cell mutagens, while the analysis of tubule germ cells has generated both positive and either negative or equivocal results.
Its function in germ cells has not yet been investigated.
Although it has been clear that piRNAs play a role in repression of retrotransposons during spermatogenesis, the function of miRNA in mouse germ cells has been unclear.
As in fetal development, dysregulation of protein expression in germ cells has dire consequences for gametogenesis.
Immunofluorescence staining of isolated testicular germ cells has revealed expression of CAR on spermatogonia, spermatocytes, round spermatids, and elongate spermatids.
Similar(37)
Male germ cells have a complex transcriptome.
TR: Does your work on gene silencing in germ cells have any applications to stem-cell research?
Consistent with K27me3 M+P− germlines adopting a neuronal identity, we readily observed patches of highly concentrated GFP signal in UNC-119 GFP UNC-119 GFPndicatingermlinese normally syncytindicatingells had cellularized.
Dazl−/− male germ cells had relatively low expression of early germ cell markers such as Pumilio-2, Stella and Oct-4, while mutant female germ cells had relatively high expression of these same markers relative to their wildtype littermates.
Germ cells have been shown to have repeat elements that are enriched in the DNA methylation associated SNPs [C/T]G or C[G/A] [55].
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