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Mutations in these genes decrease DNA damage-induced but not physiological germ cell death [47].
Moreover, GCK-1 deficient animals display a significant increase in germ cell death.
A germ cell death defect was associated with sctt maternal-/zygotic+ embryos.
Therefore nvp63 is most likely required for UV-induced germ cell death in adult N. vectensis polyps.
Indeed germ cell death by defect is a recurring phenomenon observed in a variety of bilaterian species [2].
To evaluate whether the observed BTB dysfunction following SCI was accompanied by germ cell death, TUNEL staining was carried out to detect apoptotic cells in the testis tissue.
MAP kinase activation in pachytene is also required for a developmentally programmed germ cell death switch that reduces the number of maturing oocytes by one half [27].
Loss of GCK-1 results in the hyper-activation of a specific MAP ERK kinase isoform, abnormal oocyte development, and increased germ cell death.
This germ cell death was dependent on the apoptotic machinery, as no dying cells were detected in ced-3 or ced-4 mutant animals (Figure 7)[27].
We observed germaria devoid of germ cells in cul-5 mutant ovarioles, which indicates either defects in GSC maintenance or induction of ectopic germ cell death.
The frequency of germ cell death in him-7 e1480) him-7 e1480n259) hermandrodites depleted of Gclk-2was significantly hermaphroditesntrol treatedepleteds (Figure 7), indicating that the increase in germ cell death in gck-1(lf) animals is nof mediated by the DNA checkpoint pathway.
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