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Family studies of extended pedigrees selected from geographical isolate can be a powerful approach in identifying rare genetic variants of complex diseases such as psychotic disorders.
B. xylophilus geographical isolate HF (from Setubal Region, Portugal) was cultured on small squared potato dextrose agar, previously covered with B. cinerea mycelium for 7 days at 26°C, placed in test tubes and incubated at 26°C.
Twenty kilobases of intergenic ptDNA-sequence data from an additional geographical isolate of C. reinhardtii CC-22900) was obtained by data mining plastid sequences from GenBank; because very little of these sequence data overlap with the 14 regions described above they were only compared to the ptDNA of CC-503.
Moreover, at 82% coding it is one of the most compact mitochondrial genomes available from green algae (for a compilation see [ 12]), and although the mtDNA of one geographical isolate of C. reinhardtii CC-13733) han an optional intron in cob [ 13] it is still > 75% coding; this strain is often referred to as Chlamydomonas smithii but is in fact a member of the C. reinhardtii species [ 14].
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The array contains probes representing genome sequences of two distinct geographical isolates (i.e. 3D7 and HB3) and sub-telomeric var gene sequences of a third isolate (IT4) known to adhere in culture condition.
The topology of these trees showed little depth of the internal branches but revealed the presence of some clades composed by geographical isolates.
Experimental quantification of transmission [30], [31] of geographical isolates under different conditions (e.g., host density, super-infection vs. co-infection) would provide a good test of this hypothesis.
Furthermore, two different geographical isolates and developmental stages have varied responses to the oriental beetle pheromone.
Thus, to directly confront these issues, we measured πsilent from the ptDNA of various geographical isolates of C. reinhardtii.
For our analysis we employed seven geographical isolates of C. reinhardtii, which are listed in Table 1.
Nevertheless, they necessarily have evolved as geographical isolates during a certain amount of time and cannot be considered as resulting from simple admixture of surrounding populations.
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