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The previous published Torradovirus genus specific RT-PCR primers were tested and shown to detect CaTV.
However, the appearance and thus degree of control directing the assembly is genus specific.
The culture contained Dehalococcoides species as determined by polymerase chain reaction (PCR) with genus specific primers.
Published 16S rRNA sequences were aligned and used to design genus specific primers (EntF and EntR).
Genus specific primer have been successfully developed for Lactobacillus, Mycoplasma, Bifidobacterium, Pandorea, Clostridium and recently for certain Bacillus strains [104].
Genus specific PCR assays were carried out using forward primer LbLMA-1 and reverse primer R-161 [85].
Similar(43)
The Bifidobacterium-genus specific PCR had less discriminative power as more control samples were positive.
The designed genus-specific primer probe systems were validated for sensitivity, specificity and amplification in the presence of background DNA.
In these cases, a genus-specific PCR assay is sufficient.
Samples from seven horses hybridized to a Theileria/Babesia genus-specific and a Theileria genus-specific probe.
We calculated unsampled species fractions using the genus-specific sampling fractions we determined for BAMM analyses.
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