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Work of our own also shows that repeated sputum samples of CF patients are not overly stable when analyzed at the individual genus distribution, yet the overall composition matched well a corresponding throat sample.
Although the exact causes of this variation remain elusive, we demonstrate that monoploid GS increases significantly towards the limits of the genus' distribution.
Future experimental studies will show whether larger GS in Knautia limits adaptive and competitive abilities of populations [ 76] at the genus' distribution margins and might thus represent a factor limiting further range expansion.
For genus distribution, 27.49% of the distinct sequences had top matches (first hit) with sequences from Arabidopsis, followed by the Oryza (21.77%), Picea (11.56%), Zea (5.16%) and Populus (3.78%).
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In our knowledge, there is no report showing long-term maintenance of RM systems leading to their cross-genus distribution.
In contrast, the ancestor of crown snakes is unequivocally reconstructed as having originated on Laurasia for the genetic tree using the no-genus distribution (86.45% North America and 13.55% North America + Asia).
Lagrange [ 54] biogeographic analysis of the 'Plate II' tectonic plate character yielded ambiguous results (see Additional file 5) for all tree topologies when the complete dataset (hereafter, the 'full-genus' distribution – whereby tip taxa were coded to represent the entire biogeographic range of the genus to which they belong in traditional taxonomies – see Methods) was used.
However, the Laurasian reconstruction for the ancestor of crown snakes using the no-genus distribution is potentially influenced by sampling bias, as our dataset contains mostly representatives of Scolecophidia from North America and the Caribbean, despite the worldwide distribution of scolecophidians.
Furthermore, the 25 baboon individuals represented all five Papio species (P. papio, P. hamadryas, P. anubis, P. cynocephalus, P. ursinus) and covered most of the genus' geographic distribution.
Second, the earliest fossil records of lowland tapir are reported for the late Pleistocene in the southern range of the genus' current distribution [ 19] indicating the range expansion of the lowland tapir was rapid.
In summary, our work depicts a current overview of the genus Listeria distribution in primary foods used as raw material in large national food industry that remain in the final product even after processing.
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