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Many enveloped RNA viruses yield particle-to-plaque-forming unit (pfu) ratios in the tens or hundreds [30], [31], [32], [33], however studies with Bunyamwera virus (Orthobunyavirus genus) determined that the particle-to-pfu ratio approaches one [34].
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Yet species and genus do not merely indicate quality, like the term 'white'whiteindicatescates quality and nothing further, but species and genus determine the quality with reference to a substance: they signify substance qualitatively differentiated.
The copy number of the 16S rRNA gene from each genus thus determined was in good agreement (Pearson correlations >0.90, P-value <0.01) with the relative abundance of the corresponding genus that was estimated by the assignment of 16S rRNA genes from the Roche 454 reads.
The functional trophic group of each Chironomid genus was determined following Coffman and Ferrington (1996), Henriques-Oliveira et al. (2003) and Galizzi et al. (2012).
In this comprehensive analysis of human commensal Neisseria genomes, we have identified the gene content of commensal Neisseria species, generated an extensive phylogenetic tree of this genus, and determined the distribution of virulence genes in its members.
In this work, the complete mitogenome of a species of the genus was determined for the first time.
After species or genus was determined, each fly was transferred with a pair of tweezers to live storage in a sterile plastic tube with 1 mL of saline.
The genus was determined based on taxonomic studies and genetic analysis of 16S rRNA by the identification services of TechnoSuruga Laboratory Co., Ltd., Shizuoka, Japan.
Using the free-ratio model, the branch leading to the Gallus genus was determined to have a high ω value (0.92) (Additional file 4), though this cannot be taken as strict evidence of positive selection.
All statistical analyses were run in R. Analyses of mosquito distribution were performed at genus level (Aedes n = 169, Anopheles n = 357, Culex n = 2958, Uranotaenia n = 1492, and others n = 919 (composed of Aedomyia n = 14, Coquilletidia n = 2, Culiseta n = 1, Culicinae n = 39, Eretmapodites n = 1, Harpagomyia n = 10, Mansonia n = 10, Toxorhychitinae, and genus not determined n = 845); Table 1).
Primers designed in one species may not be widely applicable across the genus when determining parental origins of a hybrid taxon.
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