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This would be due to the accumulation of errors which become more difficult to distinguish from genuine sequence, and then prevent the Rock Band algorithm from resolving repeats.
The genuine sequence tags should be mapped to the virtual tags at the forward direction.
Third, in cases of mismapping of sequences, paralogous regions that have genuine sequence differences may be mistaken for contamination.
The "modified Fibonacci-sequence" gatheterogeneousneous variation of the genuine sequence, which does not tend to a constant number at higher dose-levels.
Patient haplogroup distribution (Supplementary Table S4 online) was not significantly different from that of UK controls, suggesting that the SNV calls from WES appear to be genuine sequence variants and can be used to determine a patient's mitochondrial haplogroup.
Nonetheless, since these results were based on simulation data, which does not include the effects of any sequencing artifacts on INDEL calling, these values establish the upper bound of accuracy and performance compared to genuine sequence data.
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Thus, AT-rich sequences may display structural features favourable for Chd1-DBD binding; however, they are unlikely to be a genuine sequence-specific target in vivo.
When choosing a Phred setting one has to balance the need for high quality sequence against that of losing genuine sequences.
Increasing cutoff values, however, may also be associated with an increased risk of improperly excluding genuine sequences from the data set.
Since the genomic sequences do not have 100%% coverage and accuracy, some of the filtered sequences might be genuine sequences but did not correspond with the genomic sequence draft.
Analysis of sequences of a similar length to SAGE ditags led Prosdocimi et al. [ 48] to conclude that low Phred settings allowed the optimal ratio of genuine sequences retained to errors removed.
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