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In summary, two independent genuine mouse iPS cell lines were generated from neonatal NPCs on HFF cells in the absence of exogenous LIF.
Taken together, the data describe the first genuine mouse iPS cell lines generated on human feeder cells without exogenous LIF, providing a reliable tool for understanding the molecular mechanisms of nuclear reprogramming.
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This could also be the explanation that only CNS T-cells deficient of PD-1 show markedly enhanced INF-γ secretion after stimulation, whereas CNS CD8+ cells from genuine PLPtg mice show no relevant production of this proinflammatory cytokine.
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In both mutant lines, the more intense slower-migrating band identified as phosphorylated Parkin in phos-tag PAGE was absent (Fig. 1C, a red asterisk), suggesting that Ser65 is the genuine Parkin phosphorylation site in mouse primary neurons.
Lack of nicotine conditioned place preference in the mutant mice could have resulted from either the genuine lack of nicotine addiction or mice's inability to recognize and remember the contextual cues.
While the sequential application of two marking enzymes and two to three releasing enzymes is suitable for cDNA-AFLP profiling in A. thaliana, human, and mice, simulations on genuine EST sequences are recommended for optimizing the cDNA-AFLP strategy for organisms with different transcriptome characteristics.
The genetic sequence of XMRV in patients was nearly identical to that in patients with prostate cancer, indicating that the identified retrovirus is a genuine human virus rather than a mouse leukaemia virus contamination.
Given that the RNA-seq data we used was from tissue-matched human and mouse ENCODE libraries, this disparity appears genuine.
There are face-offs between free speech and censorship, faith and reason, passion and rationalism, all of them eloquently elaborated and set running, like so many clockwork mice, by a writer fired with genuine curiosity.
Moreover, Maestro appears to be a genuine single-copy gene, with no close paralogues existing in mice.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com