Abruptly stop other stimulation and gently squeeze your thumb and forefinger around the part of the penis where the glans meets the shaft (or your partner could do this).
5 s before the onset of the visual stimulation, flies were gently tapped into the vial on the corresponding apparatus.
After stimulation, cells were gently washed once with DMEM and incubated with monoclonal antibodies to ICAM-1 (1 200; santa cruz biotechnology, Santa Cruz, CA, USA), E-selectin and VCAM-1 (1 200; Abd serotec) for 1.5 h on ice.
Our study shows that mechanical stimulation applied by gently touching the surface of a plant cell with a glass or tungsten needle induces the same subcellular reorganization observed following inoculation of a wide range of plant species with non-adapted, avirulent and virulent fungal or oomycete pathogens.
The siphon stimulation test (SST) involved gently touching the rim of either the oral or atrial siphon with large blunt forceps (Fig. 3a).
They were fixed with tape on a smooth table to prevent movement artefacts due to the electrical stimulation, the lower limbs gently stretched to make it easier to measure distances between proximal and distal points of stimulation.
Minimize unnecessary stimulation - handle the rodent gently, don't shine bright lights at it, and don't make loud noises.
Minimize unnecessary stimulation - handle the rodent gently, don't shine bright lights at it, and don't make loud noises Agitating the rodent can cause it struggle, flee, or fight.
Massage slowly and gently to induce relaxation, and massage more quickly but deeply to induce stimulation.
Adherent macrophages or monocytes were gently washed with, and cultured in, serum-free OPTI-MEM I medium two hours before stimulation with bacterial endotoxin (lipopolysaccharide, LPS, E. coli 0111 B4, Sigma-Aldrich).
