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Gently replace chicks and eggs back.
Once you have used your ghost-detection apparatus to confirm that the spirit has entered the container, slowly and gently replace the lid, then sprinkle a pinch of salt on top.
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I lay the roots in the hole, gently replacing the soil around them and watering well with a diluted seaweed solution, which seems to reduce transplant shock.
After ∼24 hours of growth in the microscope, growth medium (2 ml) was gently replaced by growth medium with α-amanitin (100 µg/ml in 2 ml) under the microscope.
The uterus was gently replaced, the incision was closed, and normal development was allowed to continue.
After 24 hours incubation, the medium was removed and gently replaced with 2 mL of DMEM supplemented with 10% FBS.
Every two days, 1 ml of culture supernatant was gently replaced with 1 ml of the fresh medium supplemented with IL-2 (Millipore).
Every two days, 1 ml of culture supernatant was gently replaced with 1 ml of the fresh medium supplemented with IL-2 (50 U/ml, Millipore).
The medium with GM-CSF and IL-4 was gently replaced on day 2 and 4. Human recombinant GM-CSF was a kind gift from Kirin (Tokyo, Japan).
The plethora of information on treatment and the prevailing clinical perspective should be gently replaced or, at least, balanced by an effort to bring a public health perspective in mental health.
In some combined recordings experiments the culture medium of cortical preparations growing on MEA dishes at 25 to 29 Days in vitro (DIV) was gently replaced by Tyrode's physiological solution (NaCl 119 mM, KCl 2.5 mM, MgCl2 2 mM, HEPES 25 mM, CaCl2 2 mM and glucose 30 mM, pH 7.4) whereas in others, the original media was not replaced.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com