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The hindbrains were gently mounted and visualised on Olympus OV1000 with 10× or 60× objective (1024×1024).
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Utricle and cochlea sensory epithelia were gently dissected and mounted as previously described [21], [22].
After three washes in PBS, filters were gently removed and mounted on microscope slides.
The membrane was gently removed and mounted on a glass slide.
The retina was gently teased apart using mounted needles and the preparation covered with a 22 × 22 mm No. 0 glass coverslip.
It is soaked for about 20 min and gently agitated by mounting the tray on a shaker.
Coverslips were gently rinsed with PBS and mounted with ProLong gold antifade reagent (Invitrogen), followed by visualization using a Zeiss LSM 510 confocal scanning laser microscope.
Finally, the slides were rinsed gently with distilled water and mounted.
They were then gently removed from their wells, mounted on a stub with carbon tape, and examined wet using a JEOL variable pressure scanning electron microscope JSM-6460LV aroundund 50 Pa and a voltage of 10 kV.
Coverslips were washed gently in PBS and immediately dry mounted to glass slides and viewed as noted above.
Colons were gently flushed with phosphate buffer saline, mounted on bibulous paper, and splayed open along the longitudinal axis.
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