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RNAfectin mix was added to the siRNA tube and gently mixed.
Then, aqueous solution of AgNO3 (0.5 mL, 2.5 mM) was added and gently mixed.
Ingredients were added in different order to keep acid and base apart, and gently mixed.
Then, Au ions were introduced into the solution and gently mixed.
Then, the two solutions were gently mixed to get a clear solution.
The suspensions were then gently mixed with S. enterica or L. monocytogenes and incubated overnight (18 h) at room temperature.
After sampling, the reaction vessel was gently mixed to get a homogeneous suspension and the reaction was continued.
The above reaction mixture was gently mixed and incubated for 3 h with gentle and continuous vortexing.
The DNA complex was gently mixed and incubated for 45 minutes at room temperature.
Then we added 250 µl of Solution A (trypsin buffer) to the tube and gently mixed.
Cells were gently mixed and incubated at 37°C in 5% CO2 for 24 h.
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