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Wild-type and mutant hRNase3 were collected from inclusion bodies that were refolded by dialysis in refolding buffer (20 mM Tris, 0.5 M arginine, 0.2 mM GSSG, 2 mM EDTA, 10% glycerol, pH 8.5) at 4°C gently, concentrated by Amicon Ultra-15 (Millipore), and stored in phosphate-buffered saline (PBS).
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Scrub off the paste gently, concentrating more on the T-zone areas.
The volume of the buffer and the cells was taken into account, and the salt concentration was increased to 400 mM by gently adding concentrated 5 M NaCl dropwise while mixing.
Brush gently and concentrate on the outsides of the teeth.
After a single-cell suspension was prepared, the cells were collected as a crude concentrated cell suspension to which the nanoparticles were added and gently mixed.
For example: If you want to concentrate, focus gently on an object such as a coloured disc of paper, a bowl of water, a flower, a candle, or a picture of something that inspires you.
The tea bags were washed gently by filling them with distilled water and shaken in a way that concentrated the eggs at the bottom of the bags.
One of the special features of eastern Arabia is the sabkhah (or sebkha; saline flat), which usually is a gently sloping sandy plain located along the coast on which salt brine has been concentrated at or just below the surface.
How concentrated?
He concentrated.
Instead of this, gently peel the skin away from the breasts and around the thighs of the bird, as this is where much of the fat is concentrated.
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CEO of Professional Science Editing for Scientists @ prosciediting.com