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His frail, searching voice alternates with the imperious tones of the Opener (Julian Rozzell Jr). while the sound designer, Steven Leffue, offers spare, softly dissonant punctuation on piano and gentle washes of ocean waves.
At the end of the assay period, cultures were rinsed with two gentle washes with PBS (with Ca/Mg), fixed for 15 minutes with 4% paraformaldehyde and stained for 15 minutes with 0.5% Crystal Violet.
Then, three gentle washes were performed with cold PBS and incubated for 2 h with 40 µl of a 1∶10,000 dilution of goat anti-rabbit IgG (H+L) Alexa Fluor conjugate (Invitrogen, California, USA) and resuspended with 800 ml (final volume) of PBS.
Purified monocytes were obtained by adhesion; non-adherent cells (mainly lymphocytes) were removed by gentle washes with phosphate buffer.
Residual cells and ENPs are rinsed from macrowells and agarose using three gentle washes of warm phosphate buffered saline.
For the post-treatment assays Vero cells monolayers were infected with HSV-2 for two hours at 37°C, followed by two gentle washes to remove unbound viruses.
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lauramercier.com Golden delicious Forget the you've been Tango'd, sun-baked look of old: this summer, a gentle wash of colour and clever use of contouring are used in conjunction to create something altogether more flattering.
For all those other times there's this: Aesop's gentle wash cleanses and deodorises your canine pal's fur leaving them smelling of lemons, tea tree oil and spearmint.
Using this method, we demonstrated for field-grown lettuce plants that even a gentle washing protocol, designed to recover the microbial community and its metagenome from the leaf surface, resulted in substantial contamination with chloroplast DNA.
Gentle washing after 1 hour eliminated non-adherent cells.
After adsorption, unbound viruses were removed by gentle washing with PBS.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com