His voice twangs at a gentle volume over the loudspeakers, broadcast from his own Sirius radio station, and his Land Shark Lager has pride of promotional place at the cash-only bar, where a sign quotes him: "It's Five O'Clock Somewhere". One can also order a selection of seafood and sandwich options — including cheeseburgers — and, of course, drinks.
His voice twangs at a gentle volume over the loudspeakers, broadcast from his own Sirius radio station, and his Land Shark Lager has pride of promotional place at the cash-only bar, where a sign quotes him: "It's Five O'Clock Somewhere".
.... So, as Bob Caviness says in the preface of the book, "Gentle reader, I recommend this volume and all its concepts, symbols, and algorihtms to you". "This encyclopaedic book is a wonderful source of information on computer algebra.
The cocoa-leaf readers see a future of consolidation, limited innovation and a gentle decline in sales volume.
But the real highlight was "Amériques," usually a barnstormer — or, really, a tornado that hurls a barn across a field — but here offered as a subtle essay in the distance between brutal volume and gentle delicacy.
The latest volley in the decluttering business comes from Stockholm, where 80-ish artist Margareta Magnusson has just published a slim yet sage volume, "The Gentle Art of Swedish Death Cleaning". The book will be published in the United States in January.
Amid the suspicion there is tenderness too, an abortive bash at lovemaking depicted with a frank and gentle quality that speaks volumes about the couple's shared sexual past.
We have scaled down the reaction volumes for gentle pretreatment, partial enzymatic hydrolysis and sugar determination, to allow large numbers to be assessed rapidly in an automated system.
Cell nuclei were resuspended in buffer A, centrifuged (1000× g, 10 min, 4 °C) and opened by gentle homogenization with three volumes of buffer A in Dounce homogenizer.
Culture tubes were homogenized through gentle vortexing, and appropriate volumes of cells were transferred into the five replicate tubes in order to create a density of 300 000 cells/ml in tubes with 500 μl medium.
Then, samples were incubated for 1 h at room temperature with gentle agitation in 2 volumes of Ripa buffer (50 mm Tris HCl pH 8, 150 mm NaCl, 1% Triton X-100, 0.1% SDS, 0.5% sodium deoxycholate and 1 mm EDTA), containing 0.01% digitonin.
