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Samples for TEM characterization were made by a gentle mechanical grinding method and focused ion beam (FIB) milling.
In this study, we used gentle mechanical deformation of developing zebrafish embryos to probe the role of physical forces in generating long-range intercellular coordination during epiboly, the process in which the blastoderm spreads over the yolk cell.
A gentle mechanical force pushed by the nanowire probe was exerted to the tip of Au nanowire, controlled by TEM-STM manipulator.
After perfusion, lungs were collected and processed by gentle mechanical disruption with forceps followed by enzymatic digestion with 2.4 U/mL dispase gradeII (Roche), 0.25 U/mL liberase TM Research Grade medium thermolysin (Roche), 20 Kunitz units/mL DNase (Sigma), and 20 U/mL hyaluronidase (Sigma), all dissolved in PBS.
Single cell suspensions of whole spleens were obtained by gentle mechanical disruption and ACK lysis (Gibco).
The lysate was incubated at 4°C, with gentle mechanical agitation, for one hour.
Similar(22)
Therefore, their removal could be easily performed with a gentle use of soft mechanical action, like the use of a soft brush, without involving invasive methods.
In some cases units were also tested with gentle moving mechanical stimulation (brushing) to define unit type.
The reason of gentle decrease of mechanical COT is considered as the changing of the stride.
Neurons were separated by gentle agitation and mechanical treatment with a fire-polished Pasteur pipette.
Arabidopsis can detect and convert gentle forms of mechanical stimulation into a strong activation of defense against the virulent fungus B. cinerea.
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CEO of Professional Science Editing for Scientists @ prosciediting.com