The university's botanical garden offers a kinder, gentler concentration of green.
Vanillic acid bioconversion to vanillin occurred under the conditions of reduced dissolved oxygen concentration, gentle agitation, high carbon dioxide production and low specific growth rate.
This article presents an efficient, high throughput compatible method based on photometric turbidity measurements which allows a gentle and controlled concentration of the solution while constantly analyzing the solution state.
Suddenly a soundtrack of silent concentration and gentle chopping doesn't seem so bad after all.
Then, Tris-acetate buffer (500 mM, pH 8.2) and 100 μL NaCl (1 M) was added to the tubes dropwise with gentle shaking until the concentration of Tris-acetate reached 5 mM.
Volumes of protein and oleic acid (OA) stock solution were added stepwise directly to the cuvette with gentle mixing (cumulative protein concentration 1, 5, 10, 20, 40, 60 and 80 mM).
Cellular content of tissue was cleared by extracting tissue in 0.25% (v/v) White King™ bleach (4% (v/v) effective hypochlorite concentration) with gentle shaking at room temperature for at least 2 h depending on the tissue type.
According to the philosophy of traditional Chinese medicine, qigong exercise aims to achieve a harmonious flow of vital energy (qi) and regulate the functional activities of the body through regulated breathing, mindful concentration, and gentle movements.
The trend roughly follows a Langmuir isotherm adsorption model as most commonly observed in chemiresistive sensors [30], a very fast linear increase in the low-concentration region, followed by a gentle slope in the high-concentration region.
When compared to the expressed protein ligation (EPL) method [17], the trans-splicing method of this study does not require a Cys residue at the site of the protein-peptide ligation, and it is completely an enzymatic reaction that can work with very low protein concentrations under gentle or physiological conditions.
