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SNP sites present only in resistant or only in susceptible seedlings were considered the highest priority SNP sites for genotyping verification to identify resistant trait-associated DNA markers.
SNPs in those WWP ORF sequences that showed best match to P. taeda and PGI databases at protein level by BLAST search were considered for SNP genotyping verification.
Similar(58)
Genotype verification of all obtained phenotypes was done by PCR and Western blot analysis.
Synthetic media (SD) lacking specific nutrient supplement were utilized for genotype verification and selection of transformants [71].
Genotype verification of all obtained phenotypes was done by PCR and Western blot analyses using rabbit anti-YopJ/P polyclonal antibodies [10].
Of the 230 samples processed, 12% were subjected to PCR-RFLP for genotype verification.
Genotype verification was performed by conventional PCR using primers listed in Table 1.
It thus predicts the high statistical power of using this set of SNPs for tea genotype verification.
The SNP500Cancer database, a part of the Cancer Genome Anatomy Project (CGAP) (http://cgap.nci.nih.gov/Tools), is a repository for sequence and genotype verification of single nucleotide polymorphisms (SNPs) in cancer and other complex diseases [4].
The genome DNA samples were extracted from blood of the live progeny and related adult birds, and subjected to microsatellite analysis for genotyping and parentage verification.
Genotyping and verification of these targeting events was performed by PCR.
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