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The relative contribution to the resistance phenotype of the two loci tc_ rph1 and tc_ rph2 was estimated by genotyping surviving F4 individuals of the cross SICS/SRA exposed to a range of phosphine concentrations.
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Four populations from the large garden were chosen based upon their dispersed geographical origin with 7 8 genotypes per population and up to eight clones per genotype surviving the propagation technique (Table S1).
Mice of all genotypes survived normally after birth and up to 6 months of age in our vivarium.
The results showed that four genotypes survived, where two genotypes are catalytic, while the other two are non-catalytic (parasitic).
Under these conditions, only dispersive genotypes survive (and thrive) and low population sizes are overcome (Fig. 4).
Surprisingly, the Japanese genotype survived even in 1.5 M NaCl, a concentration 3-fold higher than seawater salinity.
Not all genotypes survived to age 81 day, and in some cases at later ages, only one sample of three flies per genotype and sex was collected.
The maximum salinity concentrations in which the other genotypes survived were not determined, but the Japanese genotype was not at all inferior to the other genotypes in the level of salt it could withstand.
Pairwise comparisons showed that both parental genotypes survived significantly worse than the three hybrid genotypes (all P < 0.001), whereas there were no differences in comparisons between LL and RR and LLR, LR and LRR, respectively (all P = 1.000).
If an MHC genotype survived better than the family average it would be assigned a positive score, while an MHC genotype with lower survival would get a negative one.
To understand why some hybrid genotypes survive in high salt environments, we are currently developing a modified RAD-tag sequencing method to elucidate the trait architecture of stress tolerance and to determine the karyotypes of F2 hybrids.
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