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Microarrays have been used extensively in gene expression profiling and genotyping studies.
CMC-based mutation prediction may aid design and execution of direct association genotyping studies.
Genotyping studies with microsatellites generally preferred dinucleotide repeats because they bring off high polymorphism and display more variation among individuals.
The new algorithm may therefore be useful for development of improved genotyping studies for other viruses and possibly other microorganisms.
Finally, the microfluidic chip-based method may be used for both large- and small-scale genotyping studies.
Taking into account assay performance, cost, and ease of use, method N96 has been chosen as our method of choice for future genotyping studies.
Using a single pyrosequence instrument, complete typing of HLA-DRB genes can be performed daily on hundreds of individuals for high resolution histocompatibility genotyping studies.
HRM has the potential to complement classical sequence-based methods and facilitate a wide range of genotyping studies.
Our genotyping studies showed that the LH genotype is associated with stronger DC-SIGN binding than the LL and the HH genotype.
However available genotyping studies of our peptide sequences in parasite isolates of worldwide origin indicate very limited polymorphism (PlasmoDB 5.2, and unpublished sequencing data).
However, rare CNVs (MAF<10%) are poorly tagged by SNPs and may have been missed in SNP-based GWAS, which encourages further CNV genotyping studies.
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