In this case study, little extra genetic gain could be obtained by extending the MOET scheme size with the initial genotyping strategy (800 genotyped females).
To accelerate the genotyping process, we also devised an allele-specific PCR genotyping strategy, amplifying a 414-bp wild-type band and a 800-bp mutant band (Figure 1E).
Thus, presented as a detailed case study that should be helpful to the non-specialists, we focus on the genotyping strategy to anticipate the various possibilities.
A Taqman Assay-by-Design SNP genotyping strategy was utilized to establish whether G2019S was found in association with GBA mutations.
The emergence of next-generation sequencing technologies and multiplexed sequencing methods opened the possibility to develop a new high-throughput genotyping strategy that utilizes single nucleotide polymorphisms (SNPs) generated by whole-genome sequencing (Craig et al. 2008; Cronn et al. 2008).
We also devised a PCR genotyping strategy.
The genotyping strategy utilized the Dde I restriction site generated by the ob mutation.
We utilized a PCR genotyping strategy to detect the wild type and disrupted alleles of Sulf1 and Sulf2.
Prep1i/i mice and embryos, as well as the PCR genotyping strategy, have been described previously [2] [4].
Using the SNP genotyping strategy described above, less than 10% of the lineages within haplogroup H could not be allocated to some of the already known H sub-branches (Table S3).
At a population level, the coding region genotyping strategy presented here represents a way to overcome the drawback of whole genome genotyping and allow at the same time obtaining high resolution information from the mtDNA genome.
