Sentence examples for genotyping steps from inspiring English sources

Either in this step or in later, updated, genotyping steps, multiple variable sites within a single scaffold can be combined to infer more accurate genotypes.

We next repeated the clonal isolation and genotyping steps, as above, to identify the haploid products of meiosis.

For all five loci, reproducibility was checked by performing the amplification and genotyping steps twice on 38 samples.

Primer pairs that showed an amplicon size greater than 400 bp were removed to avoid the amplification of intronic regions in subsequent genotyping steps.

We then repeated the clonal isolation and genotyping steps as above for 154 clonal isolates, of which 150 were haploid progeny (97%).

Reproducibility was checked by performing the amplification and genotyping steps on 30 samples twice, leading to 30×9 = 270 sample × locus duplicates.

Ad hoc qPCR experiments would confirm or refute the existence of the variants that failed NimbleGen validation and also determine the accuracy of the genotyping step.

The genotype of the beads was verified by a dye switch experiment, in which the original mutant-specific and wild type-specific dyes were switched for a second bead genotyping step.

This procedure identified the most informative individuals in terms of complementary recombination breakpoints from each parent, optimizing the linkage information to be extracted from these individuals and thus reducing the costs of the SFP genotyping step for map construction.

No Ct-DT detection with a Ct-DNA enzyme immunoassay was performed between the amplification and genotyping step, since all samples were already previously determined as Ct positive by the Aptima Combo 2 assay.

For the screening genotyping step the inclusion criteria were: self identified AA ESKD patients, with place of birth of the subject and both parents in the U.S. Individuals with the following diagnoses were excluded: etiology of ESKD due to polycystic kidney disease, obstructive uropathy, amyloidosis, cancer related renal failure.