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General schematic scheme for QTL introgression and pyramiding program, phenotyping and genotyping screening.
The general schematic scheme followed for QTL introgression and pyramiding program, phenotyping and genotyping screening is shown in Additional file 1: Figure S1.
The conventional breeding approach involving a series of phenotyping and genotyping screening of a large population to obtain desired variability and a high frequency of favorable genes in combination was earlier followed by several drought breeding program (Kumar et al. 2014).
The advancement of the classes with high mean grain yield performance in the F3 generation in addition to the MAB approach involving stepwise phenotyping and genotyping screening suggested this as being a cost/labor- and resource-effective breeding strategy.
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To increase the power to detect informative alleles, the sample set was enriched for DNAs that carried disease-associated single markers and haplotypes as determined in the initial genotyping screen.
AP carried out genotyping, screened the RH panel, constructed chromosome maps, performed map and sequence comparisons and drafted the manuscript.
MEJA and JEW coordinated the project, made the BBURH5000 panel, carried out genotyping, screened the RH panel, and drafted the manuscript.
Genotyping for screening purposes so far has not been deemed cost-effective.
All patients had to provide a blood sample for genotyping at screening to allow for appropriate classification within the predefined subpopulation.
It is expected to be an extremely useful tool for HPV genotypes screening.
A step further will also be to evaluate the NMR relaxometry for assessment of the effects of N depletion on senescence for genotypes screening for better response to such abiotic stress.
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