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These data can be used to improve allelic discrimination of other primer extension genotyping methodologies through directed use of nucleotide mismatches.
In addition, genotyping methodologies should be easily accessible at low cost and comparable and genotype data should be easily stored in databases and publicly accessed.
On the other hand, rare are the examples of automated procedures which would make it possible for this type of analysis to be carried out on a large scale, especially with the advent of high throughput genotyping methodologies now available.
Classical genotyping methodologies such as PFGE and MLST rely on the evaluation of highly conserved housekeeping genes representative of the vertical gene pool and resultantly provide insufficient resolution to predict disease phenotypes.
SNP marker technology is characterized by a large portfolio of genotyping methodologies that range from single-plex assays for interrogating individual loci to highly parallel multiplex assays, which can be used in various applications.
The mosquitoes used in this study were collected as described by Riehle et al. (2011), with full details on the origin of mosquito specimen, sample composition, and genotyping methodologies given in that publication.
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Details on the selection of candidate genes/SNPs and genotyping methodology have been described in detail elsewhere [17].
Therefore, it is difficult to attribute the inflation in GA heterozygosity to artifacts in genotyping methodology as most studies have reported widely differing frequencies using ARMS PCR [18].
DNA collection and genotyping methodology has been described elsewhere [ 6].
For additional detail on genotyping methodology, see the work of Lencz et al. (30).
For the initial discovery phase of novel genetic associations, we used a DNA pooling genotyping methodology.
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