After all, when you get down to it, biology research, cloning, genotyping, and experimentation in every area in between is all about precision.
Discrepancies about the relative importance of root uptake and root-to-shoot translocation may also be attributable to the genotypes selected for experimentation.
Mice were genotyped at least twice, once at weaning and again after experimentation, to verify genotype.
Before experimentation, all mice were genotyped as a confirmatory measure following receipt of PANKO-C57 mice from Jackson Laboratories using primers described in Materials and Methods (data not shown).
The genotype of all mice used for experimentation was confirmed by post hoc analysis of tail tissue using standard PCR techniques as previously described (McManus et al., 2005).
Models used for developmental and genetic studies must also offer rapid development, short generation time, be amenable to large scale cultivation, have small seed size for easy storage of many genotypes, and provide ample tissue for experimentation [ 1].
Cell line identity was confirmed at the time of experimentation by short tandem repeat genotyping and compared with our database of cell line short tandem repeat profiles (www.TXCCR.org).
However, the complete understanding of evolutionary history of such seed-size/weight-associated SBP TF gene during chickpea domestication needs further systematic experimentation by assaying high-throughput marker genotyping information in a larger set of contrasting genotypes at the genome and gene level.
However, this clarification of abp1 knock-out genotypes does not per se undermine all experimentation with ABP1 genetic tools.
Relatively low to medium empirical correlations of SNP-FT associations were observed across the three platforms underlining the need for multi-level experimentation for dissecting complex associations between genotypes and FT in rye.
