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We have demonstrated DNA amplification in this device, using an external peristaltic pump, and the PCR product was used with a DNA bioelectronic sensor chip (Motorola e-Sensors™) for genotyping experiment.
We then performed a high-throughput SNP genotyping experiment covering genes from 18p11.31 to 18q21.31 in a subset of 187 UK families.
For each genotyping experiment 10 ng DNA was used in a total volume of 5 µl containing 1× TaqMan® Genotyping Master Mix (Applied Biosystems).
In our GWAS genotyping experiment, following an Affymetrix guideline, we set a standard for the minimum DM call rate at 93% for a sample, considering all the SNPs in the two arrays (i.e., the 250k Nsp and 250k Sty arrays).
Primer mix should be made fresh before each genotyping experiment.
The equivalent individual genotyping experiment is given for reference.
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DNA was quantified and normalised to a concentration of 20 ng/μL for genotyping experiments.
These data are obtained from a range of resources including clinical trials, electronic medical records, personal health records, 'omics', sequencing and genotyping experiments.
HJK and JHK performed genotyping experiments.
PJR and LH performed genotyping experiments.
MC and ACP designed and performed SNPlex genotyping experiments.
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