"Genotype coverage" displays the percentage of positions in a given target with a high quality genotype determination: a genotype is determined (reference or variant) with a log likelihood score > =10.
Further study to confirm these findings, investigate the role of other CYP2D6 variants, and determine the clinical ramifications of these variants is needed before CYP2D6 genotype determination should become a routine test to determine tamoxifen use [ 10].
For SNPs, those genotyping technologies with redundant data sampling for SNP genotype determination, such as minisequencing [ 22], the Golden Gate™ assay [ 23] or the GeneChip® variant detection array [ 24], may be more resistant to SNP genotype failure when genotyping wgaDNA [ 9, 25, 26] than those SNP genotyping technologies with single data point genotype determination [ 27].
This design allows multiple genotype determination under an ID sequence-dependent nucleotide dispensation order during pyrosequencing.
The accuracy of E/e or e/e Rh genotype determination from the positive cell fractions was compared with that of E/e or e/e Rh genotype determination from the negative fractions.
The ANMC direct sequencing method for genotype determination compared moderately to very well with both the RFLP (84 86%) and INNO-LiPA (85 97.5%) methods.
Information on sample size, study design, Hardy Weinberg equilibrium, method of genotype determination, plasma folate and homocysteine were abstracted by two reviewers using a standardized protocol.
Information on sample size, study design, Hardy Weinberg equilibrium, and method of genotype determination was abstracted by two reviewers using a standardized protocol.
Practical diagnostic applications of such assays demonstrated in this study include: (1) rapid genotype determination of isolates; and (2) identification of mixed-genotype isolates originating from pooled samples in areas where genotype distribution is known to overlap.
Genotype determination in patients co-infected with HCV and HIV should be performed by methods relying on sequence analysis, since serotyping assays have shown a lower sensitivity in co-infected cohorts.
