Three to seven images were taken for each genotype, and quantification was performed using the ImageJ software.
In this study, quantitative PCR assays were designed for genotype-specific quantification of four epidemiologically important genotypes, GII.3, GII.4, GII.6, and GII.17.
Joshi et al. have successfully used this method for detection, genotyping, and quantification of the viral RNA of the Newcastle disease virus.
Immunocytochemistry with DILP2 antiserum was performed on adult brains from starved and fed flies of different genotypes for quantification of immunofluorescence in IPCs.
The use of PCR for pathogens detection, genotyping and quantification has some advantages, such as high sensitivity, high specificity, reproducibility and technical ease.
Owing to the texture and small size of the thymus in these genotypes, exact quantification of size or mass was not feasible.
In order to solve this problem, a multiplex real time assay was designed for simultaneous genotyping and quantification of the 18 most frequent cancer related HR-HPV types and 3 LR-HPV types of HPV-6, −11 and −81.
The combination of genome-wide genotyping with quantification of mRNA transcripts using microarray technology in sufficiently large cohorts has already demonstrated the widespread presence of eQTLs in the human genome (3– 7).
Viral sequencing, molecular HLA genotyping, and quantification of plasma VL followed procedures described in detail elsewhere (Amornkul et al. 2013; Prentice et al. 2014a; Price et al. 2011; Tang et al. 2011).
Depending on the method of determining the number of genotypes, the remaining five populations comprise between 22 and 32 genotypes; however, unambiguous quantification of the number of genotypes in these populations will require the employment of a larger number of genetic markers.
