While we found strong effects of temperature on the metabolome, genotype proved to affect an even greater number of metabolites than temperature.
First, the cadmium tolerant genotype proved to be also more tolerant to Microcystis at the organismal level, while the cadmium sensitive genotype was more sensitive to Microcystis stress (i.e., decreased reproduction after Microcystis exposure compared to a control).
The addition of only few primer pairs in genotyping proved to yield very detailed haplotype data when compared with genotypic data generated by single-SNP PCR.
Competition assays, in which antibodies were pre-incubated with one or more intact VLPs representing different genotypes, proved useful in further assessment of the antibody specificity detected by LIPS in complex polyclonal sera.
Based on the results, 37 genotypes proved to be self-compatible carrying the SC-haplotype, from which 33 were homozygous and only four were heterozygous (Table 1).
The control samples, which serve as external controls, are successfully analysed for many times during routine genotyping, proving the reproducibility of the assay.
The combined biochemical and bioinformatic protocols achieve a specificity of ~96% when based on 355,000 reads per sample (Table 4, sample indexed by CGCTA; 1 out of 27 positive genotypes proved false).
Should one particular indel region and associated genotypes prove more influential with respect to the incidence of classical BSE, differences regarding overall susceptibility and resistance for B. indicus and B. taurus cattle may be elucidated.
Although there is evidence of influence of temperature on the soybean seed oleic acid content [ 9, 10], two of our three high oleic acid soybean genotypes proved to be capable of producing a high and stable oleic acid content in three environments.
Combining RVR, HCV genotype and IL28B genotype may prove useful in guiding therapy where cost is limiting or dependent on time on therapy, particularly in the new era of DAAs (see below).
